Radiolytic Reduction of Methane Monooxygenase Dinuclear Iron Cluster at 77 K

X-ray Absorption Spectroscopic Studies of the Dinuclear Iron Center in Methane Monooxygenase and the Sulfure and Chlorine Centers in Photographic Materials*

The Diiron Center in Methane Monooxygenase. The dinuclear iron center of the hydroxylase component of soluble methane monooxygenase (MMO) from Methylococcus capsulatus (Bath) and Methylosinus trichosporium (OB3b) has been studied by X-ray absorption spectroscopy. Analysis of the Fe K-edge EXAFS revealed that the first shell coordination of the Fe(III)Fe(III) oxidized state of the hydroxylase fr...

Substrate Specificity of Soluble Methane Monooxygenase

ion or by sequential loss of a *-bond electron and a proton termed an aborted epoxidation by Ortiz de Montellano (1986) and indeed epoxidated products are generated by methane monooxygenase from these substrates (Table 111). Oxidation of Cyclopropylbenzem-Attaching a cyclopropyl ring to the hydroxylated carbon atom of a hydrocarbon substrate as a means of testing for the presence of carbon radi...

HIGH PRESSURE STUDIES OF SmSe AT 77 K

EXAFS s t u d i e s w i t h h i g h s p a t i a l r e s o l u t i o n h a v e been per formed a t t h e Se K-edge e d g e o f SmSe a t 7 7 K a n d up t o 57kbar. An i n c r e a s e i n t h e f l u c t u a t i o n o f t h e Sm-Se bond l e n g t h o f -0.05 A is o b s e r v e d upon g o i n g t h r o u g h t h e i s o s t r u c t u r a l mixed v a l e n t t r a n s i t i o n which is c o n t i n ...

The soluble methane monooxygenase gene cluster of the trichloroethylene-degrading methanotroph Methylocystis sp. strain M.

In methanotrophic bacteria, methane is oxidized to methanol by the enzyme methane monooxygenase (MMO). The soluble MMO enzyme complex from Methylocystis sp. strain M also oxidizes a wide range of aliphatic and aromatic compounds, including trichloroethylene. In this study, heterologous DNA probes from the type II methanotroph Methylosinus trichosporium OB3b were used to isolate souble MMO (sMMO...

Redox potentiometry studies of particulate methane monooxygenase: support for a trinuclear copper cluster active site.